Exons: Relevant bits of information for using the MUSC Shared Resource, the BioMolecular Computing Resource (BCR). Exon_17 Five basic things that the UNIX version of GCG can do. Start gcg: type "setgcg" and hit return. Next type "gcg" and hit return again. 1. How many pancreatic pentane synthase enzyme sequences have been reported? type this: stringsearch and hit return, then follow the prompts. 2. How can I enter my sequence and edit it? type this: seqed filename and hit return. This starts the GCG sequence editor. Enter some reference information for the sequence. Then type control-D and hit return. Next enter the letters for your sequence or "paste" the sequence text from the UNIX screen buffer. When finished, type control-D again, then type help to explore other SEQED options or type exit and then hit return to save the new sequence. 3. Is My sequence like any other known sequence? type this: fasta -check and hit return, then follow the prompts. These commands are good for nucleic acid searches. OR type this: blast -check and hit return, then follow the prompts. This command is very good for protein searches. 4. What's the restriction map of this sequence look like? type this: map -check and hit return, then follow the prompts. This command creates a text file showing the location of restriction sites for the chosen enzymes. 5. I need a primer for this region of a sequence. Can GCG design the primer for me? type this: prim and hit return, then follow the prompts.