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pepcoil |
The parallel two-stranded alpha-helical coiled coil is the most frequently encountered subunit-oligomerization motif in proteins.
pepcoil calculates the probability of a coiled-coil structure for windows of 28 residues through a protein sequence using the method of Lupas A, van Dyke M & Stock J (1991); Science 252:1162-4
% pepcoil Predicts coiled coil regions Input sequence(s): tsw:gcn4_yeast Window size [28]: Output file [gcn4_yeast.pepcoil]: |
Go to the input files for this example
Go to the output files for this example
Mandatory qualifiers:
[-sequence] seqall Sequence database USA
-window integer Window size
[-outfile] outfile Output file name
Optional qualifiers: (none)
Advanced qualifiers:
-[no]coil boolean Report coiled coil regions
-frame boolean Show coil frameshifts
-[no]other boolean Report non coiled coil regions
General qualifiers:
-help boolean Report command line options. More
information on associated and general
qualifiers can be found with -help -verbose
|
| Mandatory qualifiers | Allowed values | Default | |
|---|---|---|---|
| [-sequence] (Parameter 1) |
Sequence database USA | Readable sequence(s) | Required |
| -window | Window size | Integer from 7 to 28 | 28 |
| [-outfile] (Parameter 2) |
Output file name | Output file | <sequence>.pepcoil |
| Optional qualifiers | Allowed values | Default | |
| (none) | |||
| Advanced qualifiers | Allowed values | Default | |
| -[no]coil | Report coiled coil regions | Boolean value Yes/No | Yes |
| -frame | Show coil frameshifts | Boolean value Yes/No | Yes if -coil is true |
| -[no]other | Report non coiled coil regions | Boolean value Yes/No | Yes |
ID GCN4_YEAST STANDARD; PRT; 281 AA.
AC P03069; P03068;
DT 21-JUL-1986 (Rel. 01, Created)
DT 21-JUL-1986 (Rel. 01, Last sequence update)
DT 15-JUL-1999 (Rel. 38, Last annotation update)
DE GENERAL CONTROL PROTEIN GCN4 (AMINO ACID BIOSYNTHESIS REGULATORY
DE PROTEIN).
GN GCN4 OR ARG9 OR AAS3 OR YEL009C.
OS Saccharomyces cerevisiae (Baker's yeast).
OC Eukaryota; Fungi; Ascomycota; Hemiascomycetes; Saccharomycetales;
OC Saccharomycetaceae; Saccharomyces.
RN [1]
RP SEQUENCE FROM N.A.
RX MEDLINE; 85038531.
RA HINNEBUSCH A.G.;
RT "Evidence for translational regulation of the activator of general
RT amino acid control in yeast.";
RL Proc. Natl. Acad. Sci. U.S.A. 81:6442-6446(1984).
RN [2]
RP SEQUENCE FROM N.A.
RX MEDLINE; 84298088.
RA THIREOS G., PENN M.D., GREER H.;
RT "5' untranslated sequences are required for the translational control
RT of a yeast regulatory gene.";
RL Proc. Natl. Acad. Sci. U.S.A. 81:5096-5100(1984).
RN [3]
RP SEQUENCE FROM N.A.
RC STRAIN=S288C / AB972;
RA DIETRICH F.S., MULLIGAN J.T., HENNESSEY K.M., ALLEN E., ARAUJO R.,
RA AVILES E., BERNO A., BRENNAN T., CARPENTER J., CHEN E., CHERRY J.M.,
RA CHUNG E., DUNCAN M., GUZMAN E., HARTZELL G., HUNICKE-SMITH S.,
RA HYMAN R., KAYSER A., KOMP C., LASHKARI D., LEW H., LIN D.,
RA MOSEDALE D., NAKAHARA K., NAMATH A., NORGREN R., OEFNER P., OH C.,
RA PETEL F.X., ROBERTS D., SEHL P., SCHRAMM S., SHOGREN T., SMITH V.,
RA TAYLOR P., WEI Y., YELTON M., BOTSTEIN D., DAVIS R.W.;
RL Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP DOMAINS.
RX MEDLINE; 87002456.
RA HOPE I.A., STRUHL K.;
RT "Functional dissection of a eukaryotic transcriptional activator
RT protein, GCN4 of yeast.";
RL Cell 46:885-894(1986).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 250-281.
RX MEDLINE; 92054531.
RA O'SHEA E.K., KLEMM J.D., KIM P.S., ALBER T.;
RT "X-ray structure of the GCN4 leucine zipper, a two-stranded, parallel
RT coiled coil.";
RL Science 254:539-544(1991).
[Part of this file has been deleted for brevity]
CC -!- SUBCELLULAR LOCATION: NUCLEAR.
CC -!- SIMILARITY: TO OTHER BZIP PROTEINS.
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DR EMBL; K02205; AAA34640.1; -.
DR EMBL; K02649; AAA65521.1; -.
DR EMBL; U18530; AAB64486.1; -.
DR PIR; A03604; RGBYA1.
DR PIR; A03605; RGBYA2.
DR PDB; 1ZTA; 15-APR-93.
DR PDB; 2ZTA; 15-OCT-92.
DR PDB; 1YSA; 31-OCT-93.
DR PDB; 1DGC; 22-JUN-94.
DR PDB; 2DGC; 08-MAR-96.
DR PDB; 1GCL; 03-JUN-95.
DR PDB; 1GCM; 29-JAN-96.
DR PDB; 1SWI; 08-NOV-96.
DR PDB; 1ZII; 07-JUL-97.
DR PDB; 1ZIJ; 07-JUL-97.
DR PDB; 1ZIK; 07-JUL-97.
DR PDB; 1ZIL; 07-JUL-97.
DR PDB; 1ZIM; 07-JUL-97.
DR PDB; 1PIQ; 30-SEP-98.
DR PDB; 1CE9; 25-MAR-99.
DR TRANSFAC; T00321; -.
DR SGD; L0000683; GCN4.
DR PFAM; PF00170; bZIP; 1.
DR PROSITE; PS00036; BZIP_BASIC; 1.
KW Amino-acid biosynthesis; Transcription regulation; Activator;
KW DNA-binding; Nuclear protein; 3D-structure.
FT DOMAIN 106 125 REQUIRED FOR TRANSCRIPTIONAL ACTIVATION.
FT DNA_BIND 231 249 BASIC MOTIF.
FT DOMAIN 253 274 LEUCINE-ZIPPER.
FT CONFLICT 239 281 ARRSRARKLQRMKQLEDKVEELLSKNYHLENEVARLKKLVG
FT ER -> PGVLVRESCKE (IN REF. 2).
FT HELIX 227 276
FT TURN 277 277
SQ SEQUENCE 281 AA; 31310 MW; A2155238 CRC32;
MSEYQPSLFA LNPMGFSPLD GSKSTNENVS ASTSTAKPMV GQLIFDKFIK TEEDPIIKQD
TPSNLDFDFA LPQTATAPDA KTVLPIPELD DAVVESFFSS STDSTPMFEY ENLEDNSKEW
TSLFDNDIPV TTDDVSLADK AIESTEEVSL VPSNLEVSTT SFLPTPVLED AKLTQTRKVK
KPNSVVKKSH HVGKDDESRL DHLGVVAYNR KQRSIPLSPI VPESSDPAAL KRARNTEAAR
RSRARKLQRM KQLEDKVEEL LSKNYHLENE VARLKKLVGE R
//
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PEPCOIL of GCN4_YEAST using a window of 28 residues Other structures from 1 to 232 (232 residues) Max score: 1.283 (probability 0.21) Prediction starts at 233 Probable coiled-coil from 233 to 281 (49 residues) Max score: 1.910 (probability 1.00) |
| Program name | Description |
|---|---|
| antigenic | Finds antigenic sites in proteins |
| digest | Protein proteolytic enzyme or reagent cleavage digest |
| fuzzpro | Protein pattern search |
| fuzztran | Protein pattern search after translation |
| garnier | Predicts protein secondary structure |
| helixturnhelix | Report nucleic acid binding motifs |
| hmoment | Hydrophobic moment calculation |
| oddcomp | Finds protein sequence regions with a biased composition |
| patmatdb | Search a protein sequence with a motif |
| patmatmotifs | Search a PROSITE motif database with a protein sequence |
| pepnet | Displays proteins as a helical net |
| pepwheel | Shows protein sequences as helices |
| pestfind | Finds PEST motifs as potential proteolytic cleavage sites |
| preg | Regular expression search of a protein sequence |
| pscan | Scans proteins using PRINTS |
| sigcleave | Reports protein signal cleavage sites |
| tmap | Displays membrane spanning regions |
Original program "PEPCOIL" by Peter Rice (EGCG 1991)